کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2151199 | 1089971 | 2015 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ABCG2 Localizes to the Nucleus and Modulates CDH1 Expression in Lung Cancer Cells
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کلمات کلیدی
CSCABCG2NLSCDH1E-cadherin - E-CadherinElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزmesenchymal-epithelial transition - انتقال مزانشیمال-اپیتلیالchromatin immunoprecipitation - ایمن سازی کروماتینEMT - تکنسین فوریتهای پزشکیSide population - جمعیت جانبیcancer stem cell - سلولهای بنیادی سرطانیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکnuclear localization signal - سیگنال محلی سازی هسته ایMET - ملاقات کردCHiP - چیپEpithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Breast cancer resistance protein [BCRP/ATP-binding cassette subfamily G member 2 (ABCG2)] is a member of the ATP-binding cassette transporter family. The presence of ABCG2 on the plasma membrane in many kinds of human cancer cells contributes to multidrug resistance during chemotherapy, and it has been used as the side population marker for identifying cancer stem cells in lung cancers. We report here that, in addition to the membranous form, ABCG2 proteins are also found inside the nucleus, where they bind to the E-box of CDH1 (E-cadherin) promoter and regulate transcription of this gene. Increased expression of ABCG2 causes an increase of E-cadherin and attenuates cell migration, whereas knockdown of ABCG2 downregulates E-cadherin and enhances cell motility. In mice, xenografted A549 cells that have less ABCG2 are more likely to metastasize from the subcutaneous inoculation site to the internal organs. However, for the cancer cells that have already entered the blood circulation, an increased level of ABCG2, and correspondingly increased E-cadherin, may facilitate circulating cancer cells to colonize at a distant site and form a metastatic tumor. We propose a novel role for nuclear ABCG2 that functions as a transcription regulator and participates in modulation of cancer metastasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neoplasia - Volume 17, Issue 3, March 2015, Pages 265-278
Journal: Neoplasia - Volume 17, Issue 3, March 2015, Pages 265-278
نویسندگان
Shu-Ching Liang, Chih-Yung Yang, Ju-Yu Tseng, Hong-Ling Wang, Chien-Yi Tung, Hong-Wen Liu, Chin-Yau Chen, Yi-Chen Yeh, Teh-Ying Chou, Muh-Hwa Yang, Jacqueline Whang-Peng, Chi-Hung Lin,