کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2151692 | 1090014 | 2010 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trefoil Factor 3 Is Oncogenic and Mediates Anti-Estrogen Resistance in Human Mammary Carcinoma
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کلمات کلیدی
qPCRDMFSTFF3CS-FBSSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNABrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینdistant metastasis-free survival - زنده ماندن آزاد متاستاز دورTrefoil factor 3 - عامل تراوفیل 3quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We report herein that trefoil factor 3 (TFF3) is oncogenic and mediates anti-estrogen resistance in human mammary carcinoma. Forced expression of TFF3 in mammary carcinoma cells increased cell proliferation and survival, enhanced anchorage-independent growth, and promoted migration and invasion. Moreover, forced expression of TFF3 increased tumor size in xenograft models. Conversely, depletion of endogenous TFF3 with small interfering RNA (siRNA) decreased the oncogenicity and invasiveness of mammary carcinoma cells. Neutralization of secreted TFF3 by antibody promoted apoptosis, decreased cell growth in vitro, and arrested mammary carcinoma xenograft growth. TFF3 expression was significantly correlated to decreased survival of estrogen receptor (ER)-positive breast cancer patients treated with tamoxifen. Forced expression of TFF3 in mammary carcinoma cells increased ER transcriptional activity, promoted estrogen-independent growth, and produced resistance to tamoxifen and fulvestrant in vitro and to tamoxifen in xenograft models. siRNA-mediated depletion or antibody inhibition of TFF3 significantly enhanced the efficacy of antiestrogens. Increased TFF3 expression was observed in tamoxifen-resistant (TAMR) cells and antibody inhibition of TFF3 in TAMR cells improved tamoxifen sensitivity. Functional antagonism of TFF3 therefore warrants consideration as a novel therapeutic strategy for mammary carcinoma.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neoplasia - Volume 12, Issue 12, December 2010, Pages 1041-1053, IN23-IN31
Journal: Neoplasia - Volume 12, Issue 12, December 2010, Pages 1041-1053, IN23-IN31
نویسندگان
Nagarajan Kannan, Jian Kang, Xiangjun Kong, Jianzhong Tang, Jo K. Perry, Kumarasamypet M. Mohankumar, Lance D. Miller, Edison T. Liu, Hichem C. Mertani, Tao Zhu, Prudence M. Grandison, Dong-Xu Liu, Peter E. Lobie,