کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2166508 | 1091861 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Local extracellular acidification caused by Ca2+-dependent exocytosis in PC12 cells Local extracellular acidification caused by Ca2+-dependent exocytosis in PC12 cells](/preview/png/2166508.png)
SummaryExocytosis of acidic synaptic vesicles may produce local extracellular acidification, but this effect has not been measured directly and its magnitude may depend on the geometry and pH-buffering capacity of both the vesicles and the extracellular space. Here we have used SNARF dye immobilized by conjugation to dextran to measure the release of protons from PC12 cells. The PC12 cells were stimulated by exposure to depolarizing K+-rich solution and activation was verified by fluorescence measurement of intracellular Ca2+ and the release kinetics of GFP-labeled vesicles. Confocal imaging of the pH-dependent fluorescence from the immobile extracellular SNARF dye showed transient acidification around the cell bodies and neurites of activated PC12 cells. The local acidification was abolished when extracellular solution was devoid of Ca2+ or strong pH-buffering was imposed with 10 mM of HEPES. We conclude that the release of secretory vesicles induces local rises in proton concentrations that are co-released from synaptic vesicles with the primary neurotransmitter, and propose that the co-released protons may modulate the signaling in confined micro-domains of synapses.
Journal: Cell Calcium - Volume 44, Issue 2, August 2008, Pages 220–229