کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2166824 1091890 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calcium mobilization by nicotinic acid adenine dinucleotide phosphate (NAADP) in rat astrocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Calcium mobilization by nicotinic acid adenine dinucleotide phosphate (NAADP) in rat astrocytes
چکیده انگلیسی

Nicotinic acid adenine dinucleotide phosphate (NAADP) has been shown to release intracellular Ca2+ in several types of cells. We have used Ca2+-sensitive fluorescent dyes (Fura-2, Fluo-4) to measure intracellular Ca2+ in astrocytes in culture and in situ. Bath-applied NAADP elicited a reversible and concentration-dependent Ca2+ rise in up to 90% of astrocytes in culture (EC50 = 7 μM). The NAADP-evoked Ca2+ rise was maintained in the absence of extracellular Ca2+, but was suppressed after depleting the Ca2+ stores of the ER with ATP (20 μM), with cyclopiazonic acid (10 μM) or with ionomycin (5 μM). P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2′4′-disulfonic acid (PPADS, 100 μM), IP3 receptor blocker 2-aminoethoxydiphenyl borate (2-APB, 100 μM) and PLC inhibitor U73122 (10 μM) also reduced or suppressed the NAADP-evoked Ca2+ rise. NAADP still evoked a Ca2+ response after application of glycyl-l-phenylalanine-β-naphthylamide (GPN, 200 μM), which permeabilizes lysosomes, or preincubation with H+-ATPase inhibitor bafilomycin A1 (4 μM) and of p-trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP, 2 μM), that impairs mitochondrial Ca2+ handling. In acute brain slices, NAADP (10 μM) evoked Ca2+ transients in cerebellar Bergmann glial cells and in hippocampal astrocytes. Our results suggest that NAADP recruits Ca2+ from inositol 1,4,5-trisphosphate-sensitive Ca2+ stores in mammalian astrocytes, at least partly by activating metabotropic P2Y receptors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 39, Issue 2, February 2006, Pages 143–153
نویسندگان
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