Blocking IL-10 signalling at the time of immunization renders the tumour more accessible to T cell infiltration in mice

• E7 peptide/IL10 inhibitor vaccine elicits more T cells than E7 peptide/IFA.
• E7 peptide/IL10 inhibitor vaccine recruits more T cells to tumour.
• Priming with IL10 inhibitor boosting with E7 peptide/IFA elicits more T cells.

We recently reported that blockade of IL-10 signalling at the time of a human papillomavirus (HPV) long E7 peptide/LPS immunization leads to the regression of established HPV-16 immortalized tumours in mice similar to that induced by long E7 peptide/incomplete Freund’s adjuvant (IFA)-based vaccination. In this paper, we demonstrated that blockade of IL-10 signalling at the time of long E7 peptide/LPS could elicit stronger T cells responses and render the tumour more accessible for immune cell infiltration than vaccination with long E7 peptide/IFA. Furthermore, priming with long E7 peptide/LPS and IL10 signalling blockade then boosting with long E7 peptide/IFA elicits stronger CD8+ T cell responses than long E7 peptide/IFA immunization. The results suggest that priming with long E7 peptide/LPS and IL10 signalling inhibitor, then boosting with long E7 peptide/IFA elicits may lead to better HPV infection related tumour regression in clinic.