کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167008 | 1549404 | 2014 | 7 صفحه PDF | دانلود رایگان |
• Intraperitoneal CD90(+)CD45(−) cells grow with mesothelial morphology in culture.
• The cells highly express arginase I and strongly inhibit T cell activation.
• The cells downregulate CD3 ζ chain expression on activated T cells.
• CD3 ζ chain is reduced in intraperitoneal T cells than in circulating T cells.
• The CD90(+)CD45(−) cells may negatively regulate intraperitoneal immunity.
In this study, we analyzed intraperitoneal cells recovered from human samples and found that CD90(+)CD45(−) cells exist as a minor population but vigorously grow in culture, showing the morphological features of mesothelial cells (MC). Interestingly, the MC highly expressed arginase I and markedly suppressed T cell proliferation with the reduction of CD3 ζ chain expression in T cells stimulated by coated anti-CD3 mAb. The addition of nor-NOHA (500 μM), or l-arginine (1 mM) mostly restored the inhibitory effect of MC on T cell proliferation as well as the reduced expression of CD3 ζ chain. The expression level of CD3 ζ chain in T cells in the peritoneal cavity was significantly down-regulated from circulating T cells. These results suggest that intraperitoneal free MC have immunomodulatory functions through the control of l-arginine level, and thus may play significant roles in the pathogenesis of various diseases in the peritoneal cavity.
Journal: Cellular Immunology - Volume 288, Issues 1–2, March–April 2014, Pages 8–14