کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167039 | 1092303 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expansion of NK cells by engineered K562 cells co-expressing 4-1BBL and mMICA, combined with soluble IL-21
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CFAsMICATNF4-1BB ligandSoluble MICA4-1BBLIL-15RαIL-21PBMCsAPCMCsADCCcomplete Freund’s adjuvant - adjuvant دوست کاملmultiple cloning site - سایت کلونینگ چندگانهNK cell - سلول NKNatural killer cell - سلول قاتل طبیعیperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیK562 cells - سلولهای K562NK cells - سلولهای NKantigen presenting cell - سلولهای پردازنده آنتیژنtumor necrosis factor - فاکتور نکروز تومورMica - میکا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
NK cells hold promise for protecting hosts from cancer and pathogen infection through direct killing and expressing immune-regulatory cytokines. In our study, a genetically modified K562 cell line with surface expression of 4-1BBL and MICA was constructed to expand functional NK cells in vitro for further adoptive immunotherapy against cancer. After a long-term up to 21 day co-culture with newly isolated peripheral blood mononuclear cells (PBMCs) in the presence of soluble IL-21 (sIL-21), notable increase in proportion of expanded NK cells was observed, especially the CD56brightCD16+ subset. Apparent up-regulation of activating receptors CD38, CD69 and NKG2D was detected on expanded NK cells, so did inhibitory receptor CD94; the cytotoxicity of expanded NK cells against target tumor cells exceeded that of NK cells within fresh PBMCs. The intracellular staining showed expanded NK cells produced immune-regulatory IFN-γ. Taken together, we expanded NK cells with significant up-regulation of activating NKG2D and moderate enhancement of cytotoxicity, with IFN-γ producing ability and a more heterogeneous population of NK cells. These findings provide a novel perspective on expanding NK cells in vitro for further biology study and adoptive immunotherapy of NK cells against cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 290, Issue 1, July 2014, Pages 10-20
Journal: Cellular Immunology - Volume 290, Issue 1, July 2014, Pages 10-20
نویسندگان
Bo Jiang, Xuan Wu, Xi-ning Li, Xi Yang, Yulai Zhou, Haowei Yan, An-hui Wei, Weiqun Yan,