Mixture of fibroblast, epithelial and endothelial cells conditioned media induce monocyte-derived dendritic cell maturation

► When DCs matured in the presence of FEECM elicited stronger MLR. ► Expression of CD14 is decreased in particular in FEECM treated DCs. ► Expression of CD80, CD86 and HLA-DR is increased in FEECM treated DCs. ► Reduced phagocytic activity when DCs matured in the presence of FEECM. ► Addition of FEECM can be adapted for the generation of large amounts of mature DCs.