کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167248 | 1549414 | 2012 | 7 صفحه PDF | دانلود رایگان |
It is well known that adoptive transfer of donor-derived tolerogenic dendritic cells (DCs) helps to induce immune tolerance. RelB, one of NF-κB subunits, is a critical element involved in DC maturation. In the present study, our results showed tolerogenic DCs could be acquired via silencing RelB using small interfering RNA. Compared with imDCs, the tolerogenic DCs had more potent ability to inhibit mixed lymphocyte reaction (MLR) and down-regulate Th1 cytokines and prompt the production of Th2 cytokines. They both mediated immune tolerance via the increased of T cell apoptosis and generation of regulatory T cells. Administration of donor-derived tolerogenic DCs significantly prevented the allograft rejection and prolonged the survival time in a murine heart transplantation model. Our results demonstrate donor-derived, RelB-shRNA induced tolerogenic DCs can significantly induce immune tolerance in vitro and in vivo.
► The dendritic cell silenced by RelB shRNA inhibited MLR and shown the tolerogenic features.
► The mechanisms that DCs inhibited immune response were examined in vitro.
► The tolerogenic DCs prevent acute rejections in murine heart transplantation model.
Journal: Cellular Immunology - Volume 274, Issues 1–2, 2012, Pages 12–18