کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2167298 | 1092323 | 2011 | 5 صفحه PDF | دانلود رایگان |
Over-expression of megsin is associated with mesangial cell (MC) proliferation and extracellular matrix (ECM) accumulation. The underlying pathogenesis is unknown. This study demonstrate that over-expression of megsin induced incorporation of [3H]thymidine in MCs and PDGF-BB, TGF-β1 upregulation. Concentrations of PDGF-BB, TGF-β1 and type IV collagen in the culture medium of MCs transfected with megsin were higher than controls. Anti-PDGF-BB suppressed incorporation of [3H]thymidine in MCs transfected with megsin and mRNA expression of TGF-β1 in stable transformant MCs, suggesting that over-expression of megsin induces cell proliferation and ECM accumulation in MCs, upregulation of PDGF-BB and TGF-β1 is probably the main route involved in pathogenesis.
► Over-expression of megsin is associated with MC proliferation and ECM accumulation.
► The underlying pathogenesis was explored in MCs in vitro.
► We found that transfection of megsin induced MC proliferation and ECM accumulation.
► It was accompanied by secretion of PDGF-BB, TGF-β1 and type IV collagen.
► Over-expression of megsin is a cause of MC proliferation and ECM accumulation.
Journal: Cellular Immunology - Volume 271, Issue 2, 2011, Pages 413–417