کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168409 1092892 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo validation of in vitro quality tests for cryopreserved honey bee semen
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
In vivo validation of in vitro quality tests for cryopreserved honey bee semen
چکیده انگلیسی

Development of cryopreservation protocols for honey bee semen is hampered by the lack of validated laboratory tests that allow the prediction of in vivo performance of frozen-thawed semen. Here we analyzed correlations between seven in vitro tests and indicators of semen performance after insemination. These tests included measures of motility, cell conformation, and membrane permeability before and after exposure to physiochemical stress. We show that the proposed protocol for motility measurement yields results that correlate well with the number of sperm reaching the storage organ of queens (correlation coefficient ρ = 0.67) and the proportion of viable eggs in inseminated queens (ρ = 0.48). The conventional live/dead assay of membrane permeability by dual fluorescent staining and a new test based on the leakage of the glycolytic enzyme glucose-phosphate-isomerase (GPI) from damaged cells were also correlated to the number of sperm reaching the spermatheca (ρ = 0.54 and −0.61, respectively). We conclude that motility, live/dead-staining and the assay for GPI-leakage are valuable tools for the improvement of cryopreservation of honey bee semen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 65, Issue 2, October 2012, Pages 126–131
نویسندگان
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