کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2171028 1093466 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chromosomal stability of mesenchymal stromal cells during in vitro culture
ترجمه فارسی عنوان
پایداری کروموزومی سلول های استروما مزانشیمی در طی کشت در محیط کشت
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی

Background aimsMesenchymal stromal cells (MSCs) are being investigated for use in cell therapy. The extensive in vitro expansion necessary to obtain sufficient cells for clinical use increases the risk that genetically abnormal cells will arise and be propagated during cell culture. Genetic abnormalities may lead to transformation and poor performance in clinical use, and are a critical safety concern for cell therapies using MSCs.MethodsWe used spectral karyotyping (SKY) to investigate the genetic stability of human MSCs from ten donors during passaging.ResultsOur data indicate that chromosomal abnormalities exist in MSCs at early passages and can be clonally propagated. The karyotypic abnormalities observed during our study diminished during passage.ConclusionsKaryotyping of MSCs reveals characteristics which may be valuable in deciding the suitability of cells for further use. Karyotypic analysis is useful for monitoring the genetic stability of MSCs during expansion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 18, Issue 3, March 2016, Pages 336–343
نویسندگان
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