کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2171227 | 1093473 | 2015 | 6 صفحه PDF | دانلود رایگان |
Background aimsBone marrow (BM) transplantations are an accepted therapeutic strategy for hematologic conditions. In the past decades, interest for BM-derived cell therapy has extended toward the field of regenerative medicine. Irrespective of the treatment strategy, its success depends on the amount of cells available for transplantation. Both patient and procedural factors have been shown to influence the cell density of the BM aspirate. In the present study, the influence of core diameter of the BM aspiration device on cell density of the BM aspirate is studied.MethodsBM harvesting procedures performed in a clinical trial investigating the effect of BM cell therapy in patients with severe peripheral artery disease were retrospectively studied (clinicaltrials.govNCT00371371). Patients underwent BM harvesting through the use of either a 15-gauge (n = 85) or an 8-gauge (n = 75) needle. The numbers of harvested white blood cells (WBC) and CD34+ hematopoietic cells (HPC) were quantified.ResultsThe amount of WBC per milliliter of BM aspirate was significantly higher when the 8-gauge needle (27.8 × 106 WBC/mL [95%CI 25.4–30.5 × 106]) was used compared with the smaller 15-gauge core needle (20.1 × 106 WBC/mL [95% confidence interval (CI), 18.7–21.7 × 106], P < 0.001). For the amount of CD34+ HPC, a similar pattern was observed (185 × 103 HPC/mL [95% CI, 161–213 × 103]; 114 × 103 HPC/mL [95% CI, 96–134 × 103]; P < 0.001).ConclusionsThe application of a BM aspiration device with a larger core diameter is associated with an increased cell density of the BM aspiration product in patients with severe peripheral artery disease.
Journal: Cytotherapy - Volume 17, Issue 12, December 2015, Pages 1807–1812