کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172514 1093550 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Uptake and delivery of antigens by mesenchymal stromal cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Uptake and delivery of antigens by mesenchymal stromal cells
چکیده انگلیسی

Background aimsMesenchymal stromal cells (MSCs) are multipotent stem cells with immunosuppressive properties. Nevertheless, it has been previously reported that MSCs might also trigger the immune response. We studied whether MSCs may act as carriers, capturing antigens that can be endocytosed by antigen-presenting cells later on.MethodsWe measured the cellular uptake of mannose receptor-mediated fluid phase macropinocytosis, assessed as cellular uptake of fluorescein isothiocyanate-dextran, and PKH-67-labeled cell lysates as a surrogate marker for antigen capture among dendritic cells (DCs, positive control), T lymphocytes (negative control) and MSCs.ResultsAll experiments confirmed that MCSs displayed pinocytic and endocytic capacities, which were lower than those observed for DCs but significantly higher than those observed for T cells. We also demonstrated that MSCs release previously endocytosed antigens, which subsequently can be captured by DCs.ConclusionsMSCs have the ability to capture and release antigens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 15, Issue 6, June 2013, Pages 673–678
نویسندگان
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