کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172630 1093561 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
BM cells giving rise to MSC in culture have a heterogeneous CD34 and CD45 phenotype
چکیده انگلیسی

BackgroundMesenchymal stromal cells (MSC) isolated from adult human BM are characterized by their fibroblast-like morphology, adherent growth and capacity to differentiate into adipocytes, osteocytes, chondrocytes, cardiomyocytes and neuroprogenitors. After culturing these cells in vitro, they express the cell-surface molecules CD44, CD90, SH2 and SH3, and are negative for CD34 and the hematopoietic marker CD45. The aim of this study was to characterize the in vivo phenotype of MSC relative to the expression of CD34 and CD45.MethodsBM mononuclear cells were stained with Ab against both molecules and separated into the CD34+, CD34−, CD45+ CD34+, CD45high+ CD34−, CD45med,low+ CD34− and CD45− CD34− subpopulations, which were then cultured under the same conditions and analyzed for growth of MSC.ResultsA small population of MSC arose from the CD45+ CD34+ fraction, although the majority was obtained from the CD45− CD34− subpopulation. MSC from all fractions could be differentiated into adipocytes and osteocytes. In addition, MSC from the CD34+ and CD34− fractions were shown to differentiate into chondrocytes. After in vitro culture, MSC from both fractions possessed the same phenotype, which was negative for CD34 and CD45.DiscussionMSC from the CD45+ CD34+ fraction change their phenotype under in vitro conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 9, Issue 5, 2007, Pages 439–450
نویسندگان
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