کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172808 1093623 2016 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans
چکیده انگلیسی


• A mutation affecting the C. elegans homolog of the transcription factor PAX3 was identified, and is identical to a human mutation associated with Waardenburg syndrome.
• pax-3 reduction of function leads to ventral embryonic hypodermal cells adopting the fate of lateral embryonic hypodermal cell (lateral).
• pax-3 is the first gene required for specification of the ventral hypodermal fate in the C. elegans embryo.
• Unlike vertebrates or another nematode, P. pacificus, a role for pax-3 in myogenesis was not observed in this species.

The development of the single cell layer skin or hypodermis of Caenorhabditis elegans is an excellent model for understanding cell fate specification and differentiation. Early in C. elegans embryogenesis, six rows of hypodermal cells adopt dorsal, lateral or ventral fates that go on to display distinct behaviors during larval life. Several transcription factors are known that function in specifying these major hypodermal cell fates, but our knowledge of the specification of these cell types is sparse, particularly in the case of the ventral hypodermal cells, which become Vulval Precursor Cells and form the vulval opening in response to extracellular signals. Previously, the gene pvl-4 was identified in a screen for mutants with defects in vulval development. We found by whole genome sequencing that pvl-4 is the Paired-box gene pax-3, which encodes the sole PAX-3 transcription factor homolog in C. elegans. pax-3 mutants show embryonic and larval lethality, and body morphology abnormalities indicative of hypodermal cell defects. We report that pax-3 is expressed in ventral P cells and their descendants during embryogenesis and early larval stages, and that in pax-3 reduction-of-function animals the ventral P cells undergo a cell fate transformation and express several markers of the lateral seam cell fate. Furthermore, forced expression of pax-3 in the lateral hypodermal cells causes them to lose expression of seam cell markers. We propose that pax-3 functions in the ventral hypodermal cells to prevent these cells from adopting the lateral seam cell fate. pax-3 represents the first gene required for specification solely of the ventral hypodermal fate in C. elegans providing insights into cell type diversification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 412, Issue 2, 15 April 2016, Pages 191–207
نویسندگان
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