کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2172927 | 1093653 | 2014 | 15 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling](/preview/png/2172927.png)
• MACF1 is required for cortical neuron migration and positioning.
• Elimination of MACF1 disrupts the formation of leading processes.
• MACF1 regulates centrosome movement in migrating neurons.
• MACF1 controls microtubule stability and dynamics in cortical neurons.
• MACF1 is associated with GSK-3 signaling in cortical neuron migration.
Neuronal migration and subsequent differentiation play critical roles for establishing functional neural circuitry in the developing brain. However, the molecular mechanisms that regulate these processes are poorly understood. Here, we show that microtubule actin crosslinking factor 1 (MACF1) determines neuronal positioning by regulating microtubule dynamics and mediating GSK-3 signaling during brain development. First, using MACF1 floxed allele mice and in utero gene manipulation, we find that MACF1 deletion suppresses migration of cortical pyramidal neurons and results in aberrant neuronal positioning in the developing brain. The cell autonomous deficit in migration is associated with abnormal dynamics of leading processes and centrosomes. Furthermore, microtubule stability is severely damaged in neurons lacking MACF1, resulting in abnormal microtubule dynamics. Finally, MACF1 interacts with and mediates GSK-3 signaling in developing neurons. Our findings establish a cellular mechanism underlying neuronal migration and provide insights into the regulation of cytoskeleton dynamics in developing neurons.
Journal: Developmental Biology - Volume 395, Issue 1, 1 November 2014, Pages 4–18