A postmitotic function and distinct localization mechanism for centralspindlin at a stable intercellular bridge

► Centralspindlin functions to maintain gonad architecture in C. elegans development. ► CYK-4 localization to the germline rachis bridge requires anillin but not ZEN-4. ► ZEN-4 localization to the rachis bridge requires the GAP activity of CYK-4. ► Microtubule regulation by ZEN-4 mediates centralspindlin function at rachis bridge.