کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2173134 | 1093697 | 2013 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Myocardial Mycn is essential for mouse ventricular wall morphogenesis Myocardial Mycn is essential for mouse ventricular wall morphogenesis](/preview/png/2173134.png)
MYCN is a highly conserved transcription factor with multifaceted roles in development and disease. Mutations in MYCN are associated with Feingold syndrome, a developmental disorder characterized in part by congenital heart defects. Mouse models have helped elucidate MYCN functions; however its cardiac-specific roles during development remain unclear. We employed a Cre/loxp strategy to uncover the specific activities of MYCN in the developing mouse myocardium. Myocardial deletion of Mycn resulted in a thin-myocardial wall defect with dramatically reduced trabeculation. The mutant heart defects strongly resemble the phenotype caused by disruption of BMP10 and Neuregulin-1 (NRG1) signaling pathways, two central mediators of myocardial wall development. Our further examination showed that expression of MYCN is regulated by both BMP and NRG1 signaling. The thin-wall defect in mutant hearts is caused by a reduction in both cell proliferation and cell size. MYCN promotes cardiomyocyte proliferation through regulating expression of cell cycle regulators (including CCND1, CCND2, and ID2) and promotes cardiomyocyte growth through regulating expression of p70S6K. In addition, expression of multiple sarcomere proteins is altered in Mycn myocardial-inactivation embryos, indicating its essential role for proper cardiomyocyte differentiation. In summary, Mycn acts downstream of BMP and NRG1 cardiogenic signaling pathways to promote normal myocardial wall morphogenesis.
► Mycn was conditionally deleted from the developing mouse myocardium.
► Loss of Mycn caused hypocellular myocardial wall defect and embryonic lethality.
► MYCN is needed for proliferation in part through regulating Ccnd1, Ccnd2, and Id2.
► MYCN is required for cardiomyocyte size and proper expression of p70(s6k).
► Mutant myocardium had abnormal cardiac structural gene expression.
Journal: Developmental Biology - Volume 373, Issue 1, 1 January 2013, Pages 53–63