کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2176168 1093865 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A secondary disruption of the dmpA gene encoding a large membrane protein allows aggregation defective Dictyostelium rasC− cells to form multicellular structures
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A secondary disruption of the dmpA gene encoding a large membrane protein allows aggregation defective Dictyostelium rasC− cells to form multicellular structures
چکیده انگلیسی

The disruption of the gene encoding the Dictyostelium Ras subfamily protein, RasC, results in a strain that does not aggregate and has defects in both cAMP signal relay and cAMP chemotaxis. Disruption of a second gene in the rasC− strain by Restriction Enzyme Mediated Integration produced cells that were capable of forming multicellular structures in plaques on bacterial lawns. The disrupted gene (dmpA) encoded a novel membrane protein that was designated Dmp1. Although the rasC−/dmpA− cells progressed through early development, they did not form aggregation streams on a plastic surface under submerged starvation conditions. Phosphorylation of PKB in response to cAMP, which is significantly reduced in rasC− cells, remained low in the rasC−/dmpA− cells. However, in spite of this low PKB phosphorylation, the rasC−/dmpA− cells underwent efficient chemotaxis to cAMP in a spatial gradient. Cyclic AMP accumulation, which was greatly reduced in the rasC− cells, was restored in the rasC−/dmpA− strain, but cAMP relay in these cells was not apparent. These data indicate that although the rasC−/dmpA− cells were capable of associating to form multicellular structures, normal aggregative cell signaling was clearly not restored. Disruption of the dmpA gene in a wild-type background resulted in cells that exhibited a slight defect in aggregation and a more substantial defect in late development. These results indicate that, in addition to the role played by Dmp1 in aggregation, it is also involved in late development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 292, Issue 1, 1 April 2006, Pages 68–78
نویسندگان
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