کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2178985 1549770 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vacuolin, a flotillin/reggie-related protein from Dictyostelium oligomerizes for endosome association
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Vacuolin, a flotillin/reggie-related protein from Dictyostelium oligomerizes for endosome association
چکیده انگلیسی

We have analysed the domain structure of vacuolin, a Dictyostelium protein binding to the cytoplasmic surface of late endosomes. Localisation studies using GFP fusions together with a yeast two-hybrid analysis and co-immunoprecipitation experiments reveal that a region close to the C-terminus mediates oligomer formation of the protein through a coiled-coil mechanism which in turn is a prerequisite for the efficient binding to endosomal membranes via a prohibitin (PHB) domain in the middle of the molecule. Overexpression of the coiled-coil domain strongly competes with endogenous vacuolin in the oligomers and reduces the efficiency of membrane targeting. The domain arrangement of vacuolin is most similar to flotillin/reggie, a protein found on late endosomes of mammalian cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 85, Issues 9–10, 27 September 2006, Pages 991–1000
نویسندگان
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