کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2180515 | 1095176 | 2013 | 7 صفحه PDF | دانلود رایگان |
Chemical genetics provides tools for controlling the activity of a selected enzyme in a dose-dependent and reversible manner. To study kinase functions, a system has been developed, which combines an engineered variant of the enzyme with modified chemical inhibitors or ATP analogs, which are not accepted by wild-type kinases. While this system has been widely utilized in yeast research, it remains underappreciated in filamentous fungi. Therefore, this powerful tool holds a strong, mostly untapped potential for the experimental research in this important group of organisms.
► Chemical genetics provides tools to specifically inhibit a chosen kinase.
► The ATP-binding pocket of the kinase is mutated in order to accept large inhibitors.
► This system allows dose dependent and reversible inhibition of the kinase.
► The system is widely applied in yeast research.
► Chemical genetics holds great promise for filamentous fungal research.
Journal: Fungal Biology Reviews - Volume 27, Issue 1, May 2013, Pages 25–31