کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2180900 1095241 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caloric restriction restores the chronological life span of the goa1 null mutant of Candida albicans in spite of high cell levels of ROS
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Caloric restriction restores the chronological life span of the goa1 null mutant of Candida albicans in spite of high cell levels of ROS
چکیده انگلیسی

The Candida albicans Goa1p is required for mitochondrial functions. In a strain lacking GOA1 (GOA31), respiration, mitochondrial membrane potential, complex I (CI) activity of the electron transport chain, and ATP synthesis are significantly decreased. A shortened chronological life span (CLS) of GOA31 occurs in 2% glucose that is associated with an increase in cell reactive oxidant species (ROS) and apoptosis. We now show that caloric restriction (CR) in media containing 0.5% glucose instead of 2% glucose-SC extends the CLS to the level of parental and gene-reconstituted strains. Paradoxically, ROS levels in GOA31 far exceed those of control strains in 0.5% glucose and, as a consequence, increased lipid peroxidation occurs even though CLS is restored. Microarray analysis was used to characterize transcriptional changes during CR in GOA31. We found that CR shifts cells of all strains to a non-glucose carbon metabolism (β-oxidation). Our model of ROS formation in GOA31 follows the paradigm that the generation of oxygen radicals from β-oxidation of cell lipids via FADH2 (CII) and NADH (CI) creates an unfavorable cellular FADH2/NADH ratio that causes a transient overload in CII activity resulting in excess free cell radicals. In GOA31 the CI and peroxisomal dysfunctions increase the levels of ROS compared to control strains. Recovery from high levels of ROS may be associated with an increase in iron and sugar transporters, as well as an anti-stress response that includes the SOD1 and GPX1. Thus, CR creates a favorable growth environment, but cells of GOA31 must overcome a high but transient ROS production.


► We show that the GOA1 mutant of Candida albicans dies in high glucose.
► However, caloric restriction (CR) rescues mutant cells.
► During CR, ROS is much higher than control strains but cells survive.
► An up regulation of alternate carbon metabolism genes occurs during CR.
► We describe the relationship of CR and aging in a fungal pathogen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 49, Issue 12, December 2012, Pages 1023–1032
نویسندگان
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