کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2180936 1095249 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A heme peroxidase of the ascomyceteous lichen Leptogium saturninum oxidizes high-redox potential substrates
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A heme peroxidase of the ascomyceteous lichen Leptogium saturninum oxidizes high-redox potential substrates
چکیده انگلیسی

Lichens belonging to the order Peltigerales display strong activity of multi-copper oxidases (e.g. tyrosinase) as well as heme-containing peroxidases. The lichen peroxidase was purified to homogeneity from the thallus of Leptogium saturninum (LsaPOX) by fast protein liquid chromatography and then partially characterized.The oligomeric protein occurs as both 79 kDa dimeric and 42 kDa monomeric forms, and displayed broad substrate specificity. In addition to an ability to oxidize classic peroxidase substrates (e.g. 2,6-dimethoxyphenol), the enzyme could convert recalcitrant compounds such as synthetic dyes (e.g. Azure B and Reactive Blue 5), 4-nitrophenol and non-phenolic methoxylated aromatics (e.g. veratryl alcohol). Comparing LsaPOX with a basidiomycete dye-decolorizing (DyP)-type peroxidase from Auricularia auricula-judae showed that the lichen enzyme has a high-redox potential, with oxidation capabilities ranging between those of known plant and fungal peroxidases. Internal peptide fragments show homology (up to 60%) with putative proteins from free-living ascomycetes (e.g. Penicillium marneffei and Neosartorya fischeri), but not to sequences of algal or cyanobacterial peptides or to known fungal, bacterial or plant peroxidases.LsaPOX is the first heme peroxidase purified from an ascomyceteous lichen that may help the organism to successfully exploit the extreme micro-environments in which they often grow.


► First heme peroxidase of a lichenized ascomycete.
► Physicochemical and biochemical characterization of this new enzyme.
► Enzyme exhibits an unusual broad substrate spectrum.
► LsaPOX peptide shows no similarity to any known peroxidase group.
► Lichens have a high redox activity and overlooked role in the carbon cycle.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 48, Issue 12, December 2011, Pages 1139–1145
نویسندگان
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