Gliotoxin effects on fungal growth: Mechanisms and exploitation

► Gliotoxin exposure induces proteome remodelling in Aspergillus fumigatus. ► Intracellular glutathione facilitates gliotoxin sensitivity, via ROS formation. ► Gliotoxin exhibits potent antifungal effects. ► Gliotoxin resistance facilitates detection of targeted gene deletion. ► Gliotoxin may be part of the oxidant defense system in A. fumigatus.