کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181086 1095265 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel insights into the functional role of three protein arginine methyltransferases in Aspergillus nidulans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Novel insights into the functional role of three protein arginine methyltransferases in Aspergillus nidulans
چکیده انگلیسی

Protein arginine methylation has been implicated in different cellular processes including transcriptional regulation by the modification of histone proteins. Here we demonstrate significant in vitro activities and multifaceted specificities of Aspergillus protein arginine methyltransferases (PRMTs) and we provide evidence for a role of protein methylation in mechanisms of oxidative stress response. We have isolated all three Aspergillus PRMTs from fungal extracts and could assign significant histone specificity to RmtA and RmtC. In addition, both enzymes were able to methylate several non-histone proteins in chromatographic fractions. For endogenous RmtB a remarkable change in its substrate specificity compared to the recombinant enzyme form could be obtained. Phenotypic analysis of mutant strains revealed that growth of ΔrmtA and ΔrmtC strains was significantly reduced under conditions of oxidative stress. Moreover, mycelia of ΔrmtC mutants showed a significant retardation of growth under elevated temperatures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 47, Issue 6, June 2010, Pages 551–561
نویسندگان
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