کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2183942 1095604 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Susceptibility of germ-free pigs to challenge with protease mutants of Salmonella enterica serovar Typhimurium
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Susceptibility of germ-free pigs to challenge with protease mutants of Salmonella enterica serovar Typhimurium
چکیده انگلیسی

Salmonella protease mutants, clpP and especially htrA, are candidate live oral vaccines in humans. A functional and mature immune system is, however, required to cope with them in mice. Here, we test the cytokine response of highly susceptible germ-free pigs to infection with Salmonella Typhimurium clpP and htrA mutants. Cytokine levels (IL-4, IL-10, IL-18 and IFN-γ) were measured by ELISA in plasma and washes from the terminal small bowel 24 h after oral challenge. Unlike the infection with the wild type strain, no IFN-γ response and low IL-18 intestinal levels were found in pigs infected with the protease mutants. Despite this and regardless of partially reduced ability of htrA and clpP mutants to invade and multiply in a 3D4 porcine macrophage-like cell line, both the mutants were as virulent as was the wild type LT2 strain and caused fatal septicaemia in germ-free pigs. IFN-γ and IL-18 response therefore did not correlate with the virulence of Salmonella Typhimurium. Our results indicate that htrA and clpP attenuations should be used with caution in populations in which an increased number of immunocompromised individuals can be expected.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 212, Issue 7, 5 September 2007, Pages 577–582
نویسندگان
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