کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2184951 1095951 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Systematic Chromosomal Deletion of Bacterial Ribosomal Protein Genes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Systematic Chromosomal Deletion of Bacterial Ribosomal Protein Genes
چکیده انگلیسی

Detailed studies of ribosomal proteins (RPs), essential components of the protein biosynthetic machinery, have been hampered by the lack of readily accessible chromosomal deletions of the corresponding genes. Here, we report the systematic genomic deletion of 41 individual RP genes in Escherichia coli, which are not included in the Keio collection. Chromosomal copies of these genes were replaced by an antibiotic resistance gene in the presence of an inducible, easy-to-exchange plasmid-born allele. Using this knockout collection, we found nine RPs (L15, L21, L24, L27, L29, L30, L34, S9, and S17) nonessential for survival under induction conditions at various temperatures. Taken together with previous results, this analysis revealed that 22 of the 54 E. coli RP genes can be individually deleted from the genome. These strains also allow expression of truncated protein variants to probe the importance of RNA–protein interactions in functional sites of the ribosome. This set of strains should enhance in vivo studies of ribosome assembly/function and may ultimately allow systematic substitution of RPs with RNA.

Graphical AbstractFigure optionsDownload high-quality image (119 K)Download as PowerPoint slideResearch Highlights
► We deleted 41 RP genes from the E. coli chromosome.
► Deletion was complemented by an inducible, plasmid-born allele.
► Of the targeted genes, nine were identified as nonessential for survival.
► The function of L5, L27, and S19 were probed by truncation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 413, Issue 4, 4 November 2011, Pages 751–761
نویسندگان
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