کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2185244 | 1095969 | 2011 | 18 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Different Requirements for σ Region 4 in BvgA Activation of the Bordetella pertussis Promoters Pfim3 and PfhaB Different Requirements for σ Region 4 in BvgA Activation of the Bordetella pertussis Promoters Pfim3 and PfhaB](/preview/png/2185244.png)
Bordetella pertussis BvgA is a global response regulator that activates virulence genes, including adhesin-encoding fim3 and fhaB. At the fhaB promoter, PfhaB, a BvgA binding site lies immediately upstream of the − 35 promoter element recognized by Region 4 of the σ subunit of RNA polymerase (RNAP). We demonstrate that σ Region 4 is required for BvgA activation of PfhaB, a hallmark of Class II activation. In contrast, the promoter-proximal BvgA binding site at Pfim3 includes the − 35 region, which is composed of a tract of cytosines that lacks specific sequence information. We demonstrate that σ Region 4 is not required for BvgA activation at Pfim3. Nonetheless, Region 4 mutations that impair its typical interactions with core and with the − 35 DNA affect Pfim3 transcription. Hydroxyl radical cleavage using RNAP with σD581C–FeBABE positions Region 4 near the − 35 region of Pfim3; cleavage using RNAP with α276C–FeBABE or α302C–FeBABE also positions an α subunit C-terminal domain within the − 35 region, on a different helical face from the promoter-proximal BvgA~P dimer. Our results suggest that the − 35 region of Pfim3 accommodates a BvgA~P dimer, an α subunit C-terminal domain, and σ Region 4. Molecular modeling suggests how BvgA, σ Region 4, and α might coexist within this DNA in a conformation that suggests a novel mechanism of activation.
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Journal: Journal of Molecular Biology - Volume 409, Issue 5, 24 June 2011, Pages 692–709