کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2187339 1096110 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An Indigenous Posttranscriptional Modification in the Ribosomal Peptidyl Transferase Center Confers Resistance to an Array of Protein Synthesis Inhibitors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
An Indigenous Posttranscriptional Modification in the Ribosomal Peptidyl Transferase Center Confers Resistance to an Array of Protein Synthesis Inhibitors
چکیده انگلیسی

A number of nucleotide residues in ribosomal RNA (rRNA) undergo specific posttranscriptional modifications. The roles of most modifications are unclear, but their clustering in functionally important regions of rRNA suggests that they might either directly affect the activity of the ribosome or modulate its interactions with ligands. Of the 25 modified nucleotides in Escherichia coli 23S rRNA, 14 are located in the peptidyl transferase center, the main antibiotic target in the large ribosomal subunit. Since nucleotide modifications have been closely associated with both antibiotic sensitivity and antibiotic resistance, loss of some of these posttranscriptional modifications may affect the susceptibility of bacteria to antibiotics. We investigated the antibiotic sensitivity of E. coli cells in which the genes of 8 rRNA-modifying enzymes targeting the peptidyl transferase center were individually inactivated. The lack of pseudouridine at position 2504 of 23S rRNA was found to significantly increase the susceptibility of bacteria to peptidyl transferase inhibitors. Therefore, this indigenous posttranscriptional modification may have evolved as an intrinsic resistance mechanism protecting bacteria against natural antibiotics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 380, Issue 4, 18 July 2008, Pages 593–597
نویسندگان
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