کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2187593 | 1096128 | 2008 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A New Cryo-EM Single-Particle Ab Initio Reconstruction Method Visualizes Secondary Structure Elements in an ATP-Fueled AAA+ Motor A New Cryo-EM Single-Particle Ab Initio Reconstruction Method Visualizes Secondary Structure Elements in an ATP-Fueled AAA+ Motor](/preview/png/2187593.png)
The generation of ab initio three-dimensional (3D) models is a bottleneck in the studies of large macromolecular assemblies by single-particle cryo-electron microscopy. We describe here a novel method, in which established methods for two-dimensional image processing are combined with newly developed programs for joint rotational 3D alignment of a large number of class averages (RAD) and calculation of 3D volumes from aligned projections (VolRec). We demonstrate the power of the method by reconstructing an ∼ 660-kDa ATP-fueled AAA+ motor to 7.5 Å resolution, with secondary structure elements identified throughout the structure. We propose the method as a generally applicable automated strategy to obtain 3D reconstructions from unstained single particles imaged in vitreous ice.
Journal: Journal of Molecular Biology - Volume 375, Issue 4, 25 January 2008, Pages 934–947