Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; TF, trigger factor; RBD, ribosome-binding domain; PPIase, peptidyl-prolyl cis/trans-isomerase; MST, microscale thermophoresis; MD, molecular dynamics; NC, nascent chain; cryo-EM, cryo-electron microscopy; GatD, galactitol-1-phosphate dehydrogenase; RNCs,
مقالات ISI Cryo-EM، میکروسکوپ الکترونی کریو (ترجمه نشده)
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Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; SAXS, small-angle X-ray scattering; SEC, size-exclusion chromatography; EDTA, ethylenediaminetetraacetic acid; CHESS, Cornell High Energy Synchrotron Sourcekinase; dynamics; cooperativity; regulation; phosphorylation
The Assembly Pathway of an Icosahedral Single-Stranded RNA Virus Depends on the Strength of Inter-Subunit Attractions
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; CCMV, cowpea chlorotic mottle virus; ssRNA, single-stranded RNA; BMV, brome mosaic virus; VLP, virus-like particle; CP, capsid protein; wt, wild-type; EM, electron microscopy; cryo-EM, cryo-electron microscopy; ARM, arginine-rich motif; DOL, density of la
Insights into cell entry and intracellular trafficking of peptide and protein drugs provided by electron microscopy
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; CPP, cell-penetrating peptide; cryo-EM, cryo-electron microscopy; EM, electron microcopy; ET, electron tomography; HPF, high pressure freezing; immuno-EM, immuno-electron microscopy; IFM, immunofluorescence microscopy; mAb, monoclonal antibody; Tat-SPIONs
Structural Insights into Transcriptional Repression by Noncoding RNAs That Bind to Human Pol II
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; Pol II, RNA polymerase II; ncRNA, noncoding RNA; cryo-EM, cryo-electron microscopy; PIC, pre-initiation complex; yPol II, yeast Pol II; hPol II, human Pol II; CTF, contrast transfer function; PDB, Protein Data Bank; CCD, charge-coupled device; EDTA, ethyl
Optimized negative-staining electron microscopy for lipoprotein studies
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; EM, electron microscopy; NS, negative staining; OpNS, optimized negative staining; cryo-EM, cryo-electron microscopy; IPET, individual-particle electron tomography; HDL, high-density lipoprotein; LDL, low-density lipoprotein; VLDL, very low-density lipopr
Consensus among multiple approaches as a reliability measure for flexible fitting into cryo-EM data
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; Cryo-EM, cryo-electron microscopy; RDF, rigid domain fitted; NMFF, normal mode flexible fitting; MDfit, molecular dynamics flexible fittingFlexible fitting; Consensus fitting; X-ray structure; Electron microscopy; Protein Data Bank
Simple Rules for Efficient Assembly Predict the Layout of a Packaged Viral RNA
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; ssRNA, single-stranded RNA; cryo-EM, cryo-electron microscopy; 1-D, one-dimensionalssRNA virus; virus assembly; genome organization; cryo-EM; Hamiltonian path
The Staphylococcus aureus Pathogenicity Island 1 Protein gp6 Functions as an Internal Scaffold during Capsid Size Determination
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; bacteriophage; mobilization; virus assembly; NMR spectroscopy; cryo-electron microscopySaPI, Staphylococcus aureus pathogenicity island; dsDNA, double-stranded DNA; cryo-EM, cryo-electron microscopy; MS, mass spectrometry; HSQC, heteronuclear single quant
Water Molecules in the Nucleotide Binding Cleft of Actin: Effects on Subunit Conformation and Implications for ATP Hydrolysis
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; F-actin, filamentous actin; G-actin, monomeric actin; SD, subdomain; cryo-EM, cryo-electron microscopy; MD, molecular dynamics; P-loop, phosphate-binding loopmolecular dynamics; F-actin; cytoskeleton; filament; actin polymerization
Translation factor LepA contributes to tellurite resistance in Escherichia coli but plays no apparent role in the fidelity of protein synthesis
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; Ribosome; Translation; Reverse translocation; EF4; BipAGTPase, guanosine 5′-triphosphate hydrolase; mRNA, messenger RNA; tRNA, transfer RNA; cryo-EM, cryo-electron microscopy; A site, aminoacyl site; P site, peptidyl site; E site, exit site; GDPNP, guanos
The Crystal Structure of Bacteriophage HK97 gp6: Defining a Large Family of Head–Tail Connector Proteins
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; dsDNA, double-stranded DNA; ORF, open reading frame; cryo-EM, cryo-electron microscopy; TEM, transmission electron microscopy; LC–MS/MS, liquid chromatography–tandem mass spectrometry; MCP, major capsid protein; PEG, polyethylene glycolbacteriophage assem
The On–Off Switch in Regulated Myosins: Different Triggers but Related Mechanisms
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; RD, regulatory domain; RLC, regulatory light chain; HC, heavy chain; ELC, essential light chain; MLCK, myosin light-chain kinase; HMM, heavy meromyosin; cryo-EM, cryo-electron microscopy; EGTA, ethylene glycol bis(b-aminoethyl ether) N,N′-tetraacetic acid
Nucleotide-Induced Flexibility Change in Neck Linkers of Dimeric Kinesin as Detected by Distance Measurements Using Spin-Labeling EPR
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; EPR, electron paramagnetic resonance; FRET, fluorescence resonance energy transfer; AMPPNP, adenosine 5′-(β,γ-imido)triphosphate; CW, continuous wave; DEER, double electron–electron resonance; PDB, Protein Data Bank; MSL
CLIC2-RyR1 Interaction and Structural Characterization by Cryo-electron Microscopy
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; CLIC, chloride intracellular channel; RyR, ryanodine receptor; SR, sarcoplasmic reticulum; Po, open probability; To, mean open time; Tc, mean closed timeCa2+-release channel; Ca2+ signaling; chloride intracellular channe
Crystal Structures of Limulus SAP-Like Pentraxin Reveal Two Molecular Aggregations
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; PE, phosphoethanolamine; CRP, C-reactive protein; SAP, serum amyloid P component; 3D, three-dimensional; LPx, Limulus pentraxins; cryo-EM, cryo-electron microscopy; FSC, Fourier shell correlation; PC, phosphocholine; PEG, polyethylene glycol; MPD, methyl-
A New Cryo-EM Single-Particle Ab Initio Reconstruction Method Visualizes Secondary Structure Elements in an ATP-Fueled AAA+ Motor
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; 3D, three-dimensional; 2D, two-dimensional; cryo-EM, cryo-electron microscopy; PDB, Protein Data Bank; SA, simulated annealing; PCA, principal component analysis; FT, Fourier transform; CTF, contrast transfer function; FSC, Fourier shell correlation; HslU
Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; Cryo-EM, cryo-electron microscopy; DTT, dithiothreitol; LC, liquid chromatography; LSU, large subunit; MALDI, matrix-assisted laser desorption–ionization; MRP, mitochondrial ribosomal protein; MS, mass spectrometry; rRNA, ribosomal RNA; SSU, small subunit
Comparison of Fungal 80 S Ribosomes by Cryo-EM Reveals Diversity in Structure and Conformation of rRNA Expansion Segments
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; ES, expansion segment; eEF2, elongation factor 2; cryo-EM, cryo-electron microscopy; Tlan80S, 80 S ribosome from the T. lanuginosus; Scer80S, 80 S ribosome from S. cerevisiaeelongation factor 2; Thermomyces lanuginosus; 80 S; expansion segments
Observation of Intersubunit Movement of the Ribosome in Solution Using FRET
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; EF-G, elongation factor G; IF2, initiation factor 2; RF3, release factor 3; RRF ribosome, recycling factor; cryo-EM, cryo-electron microscopy; FRET, Förster resonance energy transfertranslocation; EF-G; tRNA; hybrid states
Structural analysis of vimentin and keratin intermediate filaments by cryo-electron tomography
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; CEMOVIS, cryo-electron microscopy on vitreous sections; Cryo-EM, cryo-electron microscopy; Cryo-ET, cryo-electron tomography; CTF, contrast transfer function; EM, electron microscopy; IF, intermediate filament; PSF, point-spread function; STEM, scanning t
Analysis of Structural Dynamics in the Ribosome by TLS Crystallographic Refinement
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; TLS, translation-libration-screw; PTC, peptidyl transferase center; cryo-EM, cryo-electron microscopy; SD, Shine–Dalgarnostructural dynamics; 70 S ribosome; TLS refinement; X-ray crystallography
Crosslinking of Translation Factor EF-G to Proteins of the Bacterial Ribosome Before and After Translocation
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; AzP, 4-azidophenacyl bromide; cryo-EM, cryo-electron microscopy; DSG, disuccinimidyl glutarate; EF-G, elongation factor G; EF-Tu, elongation factor Tu; GDPNP, guanosine 5′- [β,γ-imido]triphosphate; F site, factor-binding site on the ribosome; fus, fusidic
Specific Interaction between EF-G and RRF and Its Implication for GTP-Dependent Ribosome Splitting into Subunits
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; tRNA, transfer RNA; mRNA, messenger RNA; EF-G, elongation factor G; RRF, ribosome recycling factor; IF3, initiation factor 3; GTP, guanosine 5′-triphosphate; cryo-EM, cryo-electron microscopy; FSC, Fourier shell correlation; CC, correlation coefficient; r
Protein-induced Conformational Changes of RNA During the Assembly of Human Signal Recognition Particle
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; DMS, dimethylsulphate; SRP, signal recognition particle; ER, endoplasmic reticulum; cryo-EM, cryo-electron microscopy; ENU, ethylnitrosourea; GFP, green fluorescent protein; TB, Tris-boratechemical probing; signal recognition particle; 7SL RNA; conformati
The Asymmetric ATPase Cycle of the Thermosome: Elucidation of the Binding, Hydrolysis and Product-release Steps
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; SAXS, small-angle X-ray scattering; SANS, small-angle neutron scattering; PBP, phosphate binding proteinthermosome; chaperonins; cooperativity; asymmetry; ATP-ase cycle
Isolation and characterization of mitochondrial ribosomes and ribosomal subunits from Leishmania tarentolae
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; CID, collision-induced dissociation; cryo-EM, cryo-electron microscopy; DTT, dithiotreitol; LC, liquid chromatography; LSU, large subunit; MALDI, matrix-assisted laser desorption-ionization; MRP, mitochondrial ribosomal protein; MS, mass spectrometry; rRN
Three-dimensional structure and stoichiometry of Helmintosporium victoriae190S totivirus
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; 3DR, three-dimensional reconstruction; CP, capsid protein; cryo-EM, cryo-electron microscopy; ds, double-stranded; ctf, contrast transfer function; Hv190SV, Helminthosporium victoriae 190S virus; RDRP, RNA-dependent RNA polymeraseHv190SV; Double-stranded
Structure of the Blm10–20 S Proteasome Complex by Cryo-electron Microscopy. Insights into the Mechanism of Activation of Mature Yeast Proteasomes
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; WT, wild-type; ORF, open reading frame; CTF, contrast transfer function; FSC, Fourier shell correlationBlm10; PA200; proteasome activator; cryo-electron microscopy; three-dimensional reconstruction
Cryo-electron Microscopy Reveals Conserved and Divergent Features of Gag Packing in Immature Particles of Rous Sarcoma Virus and Human Immunodeficiency Virus
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM; RSV; HIV; retrovirus assembly; Gag proteinVLP, virus-like particle; NC, nucleocapsid; CA, capsid; RSV, Rous sarcoma virus; HIV, human immunodeficiency virus; cryo-EM, cryo-electron microscopy; MA, matrix; NTD, N-terminal sub-domain; CTD, C-termin
Structural Determinants of Rotavirus Subgroup Specificity Mapped by Cryo-electron Microscopy
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; capsid structure; cryo-electron microscopy; Fab fragments; image analysis; rotavirus; subgroup specificity; transcriptaseDLP, double-layered particle; TLP, triple-layered particle; mAb, monoclonal antibody; cryo-EM, cryo-electron microscopy; TCA, trichlor
Quasi-symmetry in the Cryo-EM Structure of EmrE Provides the Key to Modeling its Transmembrane Domain
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; dual topology; protein structure prediction; structural bioinformatics; cryo-EM; mechanism of actionSMR, small multidrug resistance; TM, transmembrane; cryo-EM, cryo-electron microscopy; TPP+, tetraphenylphosphonium
Following the Dynamics of Changes in Solvent Accessibility of 16 S and 23 S rRNA During Ribosomal Subunit Association Using Synchrotron-generated Hydroxyl Radicals
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; cryo-EM, cryo-electron microscopy; DMS, dimethyl sulfateribosomes; subunit interface; intersubunit bridges; chemical probing; association kinetics
Epitope Diversity of Hepatitis B Virus Capsids: Quasi-equivalent Variations in Spike Epitopes and Binding of Different Antibodies to the same Epitope
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; antibody–antigen interaction; quasi-equivalence; conformational epitope; discontinuous epitope; cryo-electron microscopyCDR, complementarity-determining region; HBV, hepatitis B virus; cryo-EM, cryo-electron microscopy; sAg, cAg, eAg, surface, core and un
The MS2 Coat Protein Shell is Likely Assembled Under Tension: A Novel Role for the MS2 Bacteriophage A Protein as Revealed by Small-angle Neutron Scattering
Keywords: Cryo-EM، میکروسکوپ الکترونی کریو; bacteriophage MS2; contrast variation; MS2 A protein; RNA ejection; small-angle neutron scatteringSANS, small-angle neutron scattering; SAXS, small-angle X-ray scattering; SLD, scattering length density; cryo-EM, cryo-electron microscopy