کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2188595 1096178 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Simple, RNA-Mediated Allosteric Switch Controls the Pathway to Formation of a T = 3 Viral Capsid
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A Simple, RNA-Mediated Allosteric Switch Controls the Pathway to Formation of a T = 3 Viral Capsid
چکیده انگلیسی

Using mass spectrometry we have detected both assembly intermediates and the final product, the T = 3 viral capsid, during reassembly of the RNA bacteriophage MS2. Assembly is only efficient when both types of quasiequivalent coat protein dimer seen in the final capsid are present in solution. NMR experiments confirm that interconversion of these conformers is allosterically regulated by sequence-specific binding of a short RNA stem–loop. Isotope pulse–chase experiments confirm that all intermediates observed are competent for further coat protein addition, i.e., they are all on the pathway to capsid formation, and that the unit of capsid growth is a coat protein dimer. The major intermediate species are dominated by stoichiometries derived from formation of the particle threefold axis, implying that there is a defined pathway toward the T = 3 shell. These results provide the first experimental evidence for a detailed mechanistic explanation of the regulation of quasiequivalent capsid assembly. They suggest a direct role for the encapsidated RNA in assembly in vivo, which is consistent with the structure of the genomic RNA within wild-type phage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 369, Issue 2, 1 June 2007, Pages 541–552
نویسندگان
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