کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2188763 | 1096184 | 2006 | 16 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Elucidation of Human Choline Kinase Crystal Structures in Complex with the Products ADP or Phosphocholine Elucidation of Human Choline Kinase Crystal Structures in Complex with the Products ADP or Phosphocholine](/preview/png/2188763.png)
Choline kinase, responsible for the phosphorylation of choline to phosphocholine as the first step of the CDP-choline pathway for the biosynthesis of phosphatidylcholine, has been recognized as a new target for anticancer therapy. Crystal structures of human choline kinase in its apo, ADP and phosphocholine-bound complexes, respectively, reveal the molecular details of the substrate binding sites. ATP binds in a cavity where residues from both the N and C-terminal lobes contribute to form a cleft, while the choline-binding site constitutes a deep hydrophobic groove in the C-terminal domain with a rim composed of negatively charged residues. Upon binding of choline, the enzyme undergoes conformational changes independently affecting the N-terminal domain and the ATP-binding loop. From this structural analysis and comparison with other kinases, and from mutagenesis data on the homologous Caenorhabditis elegans choline kinase, a model of the ternary ADP·phosphocholine complex was built that reveals the molecular basis for the phosphoryl transfer activity of this enzyme.
Journal: Journal of Molecular Biology - Volume 364, Issue 2, 24 November 2006, Pages 136–151