کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2189276 | 1550373 | 2006 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Structural Basis for Detoxification and Oxidative Stress Protection in Membranes Structural Basis for Detoxification and Oxidative Stress Protection in Membranes](/preview/png/2189276.png)
Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat microsomal glutathione transferase 1, at 3.2 Å resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.
Journal: Journal of Molecular Biology - Volume 360, Issue 5, 28 July 2006, Pages 934–945