کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2190627 1097804 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Automated imaging reveals a concentration dependent delay in reversibility of cardiac myocyte hypertrophy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Automated imaging reveals a concentration dependent delay in reversibility of cardiac myocyte hypertrophy
چکیده انگلیسی

Cardiac hypertrophy is controlled by a dense signaling network with many pathways associated with cardiac myocyte growth. New large scale methodology is required to quantitatively characterize the pathways that distinguish reversible forms of hypertrophy from irreversible forms that lead to heart failure. Our automated image acquisition method records 5 × 5 mosaic images of fluorescent protein-labeled cardiac myocytes within each well of a 96-well plate using an automated stage and focus. Post-processing algorithms automatically identify cell edges, quantify cell phenotypes, and track cells. We uniquely applied our imaging platform to study hypertrophy reversibility in a scalable cell model. Cell area changes after washout of a dose response to the α-adrenergic receptor (αAR) agonist phenylephrine (PE) showed that hypertrophy reverses at low but not high levels of α-adrenergic signaling: a reversibility delay. Perturbations with specialized αAR antagonists, a mathematical model, and live imaging of αAR localization identify the mechanism for this reversibility delay: ligand trapping with internalized PE acting on intracellular αAR's.


► Automated image acquisition and analysis approach for cardiac myocyte hypertrophy.
► Tracks changes in measurements of individual myocytes over time.
► Reversibility of hypertrophy shown in a scalable cell culture model.
► Hypertrophy reverses at low and persists at high levels of α-adrenergic signaling.
► Delay in reversal explained by a ligand trapping model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 53, Issue 2, August 2012, Pages 282–290
نویسندگان
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