کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2195529 | 1550848 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An analogue of atrial natriuretic peptide (C-ANP4-23) modulates glucose metabolism in human differentiated adipocytes
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کلمات کلیدی
AMPKNPR3DMEMFBSIL1bHPRT1GAPDHcGMPCASP1pKaGLUT4BSA - BSAcAMP - cAMPCyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicAdipocytes - آدیپوسیتbovine serum albumin - آلبومین سرم گاوfoetal bovine serum - سرم جنین گاوDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoObesity - مرض چاقیcyclic guanosine monophosphate - مونوفسفات گوانوزین چرخه ایGlucose transporter type 4 - نوع حمل گلوکز 4protein kinase A - پروتئین کیناز ANatriuretic peptide - پپتید Natriureticcaspase 1 - کاسپاز 1AMP-activated kinase - کیناز فعال AMPglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study was undertaken to investigate the effects of C-atrial natriuretic peptide (C-ANP4-23) in human adipose-derived stem cells differentiated into adipocytes over 10 days (1 μM for 4 h). The intracellular cAMP, cGMP and protein kinase A levels were determined by ELISA and gene and protein expression were determined by qRT-PCR and Western blot, respectively, in the presence or absence of C-ANP4-23. The levels of lipolysis and glucose uptake were also determined. C-ANP4-23 treatment significantly increased the intracellular cAMP levels and the gene expression of glucose transporter type 4 (GLUT4) and protein kinase, AMP-activated, alpha 1 catalytic subunit (AMPK). Western blot showed a significant increase in GLUT4 and phosphor-AMPKα levels. Importantly, the adenylate cyclase inhibitor SQ22536 abolished these effects. Additionally, C-ANP4-23 increased glucose uptake by 2-fold. Our results show that C-ANP4-23 enhances glucose metabolism and might contribute to the development of new peptide-based therapies for metabolic diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 431, 15 August 2016, Pages 101-108
Journal: Molecular and Cellular Endocrinology - Volume 431, 15 August 2016, Pages 101-108
نویسندگان
Francisco Javier Ruiz-Ojeda, Concepción MarÃa Aguilera, Azahara Iris Rupérez, Ángel Gil, Carolina Gomez-Llorente,