Inhibitory effect of Andrographolide in 3T3-L1 adipocytes differentiation through the PPARγ pathway

Andrographolide (AG), an active compound found in Andrographis paniculate Nees, has been shown to exert anti-inflammatory, anticancer and anti-hyperglycemic effects. However, its biological activities against obesity have not been reported. The purpose of this study was to investigate the effect of AG on the differentiation of 3T3-L1 preadipocytes. We found AG significantly inhibited not only on adipocyte differentiation induced by standard adipogenic agents and MDI, but also on the adipogenesis-related transcription factor, peroxisome proliferator-activated receptor γ (PPARγ), as well as the expressions of the PPARγ targeted genes, such as CD36, LPL, FAS and other adiocyte markers. Taken together, our data showed AG inhibited the early stage of adipogenic differentiation, in part via the inhibition of PPARγ-dependent mechanisms.

► Our study provides the first evidence for the inhibitory effect of andrographolide (AG) in adipocytes differentiation.
► AG inhibited early stage of adipogenic differentiation through inhibition of PPARγ pathway.
► AG is a potential candidate drug for amelioration of obesity and diabetic conditions.