کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2196720 1098847 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The juvenile hormone analogue methoprene up-regulates the Ha-RNA-binding protein
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The juvenile hormone analogue methoprene up-regulates the Ha-RNA-binding protein
چکیده انگلیسی

RNA-binding proteins (RBPs) associate with RNA in cells to form ribonucleoprotein (RNP) complexes. The RBPs are involved in various aspects of RNA metabolism, but their roles in the juvenile hormone (JH) signaling pathway are not well known. An RNA-binding protein (Ha-RBP) was obtained from Helicoverpa armigera, a lepidopteran insect, which could be up-regulated by the juvenile hormone analogue methoprene at the mRNA level. Immunohistochemistry showed that Ha-RBP was mainly distributed in cells where protein synthesis was active, and its knockdown decreased the protein levels of JH-responsive genes. Immunocytochemistry showed that Ha-RBP was located in both the nucleus and the cytoplasm of normal cells, and methoprene could promote the translocation of Ha-RBP from the nucleus to the cytoplasm. This process was mediated by the JH receptor candidate HaMet but not by the ultraspiracle protein (USP). The knockdown of HaMet by RNAi decreased the expression of Ha-RBP and blocked its translocation from the nucleus to the cytoplasm. Together these findings indicate that Ha-RBP is involved in the juvenile hormone signaling pathway and Met mediates JH signaling by regulating Ha-RBP translocation from nucleus to cytoplasm, which may allow Ha-RBP to modify protein translation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 333, Issue 2, 20 February 2011, Pages 172–180
نویسندگان
, , , , ,