کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2196818 1550942 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Macrophage-secreted factors inhibit ZAG expression and secretion by human adipocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Macrophage-secreted factors inhibit ZAG expression and secretion by human adipocytes
چکیده انگلیسی

Zinc-α2-glycoprotein (ZAG), a novel adipokine, is downregulated in adipose tissue in obesity, a state characterized by increased adipose tissue macrophage infiltration and chronic low-grade inflammation. This study investigated whether macrophage-secreted factors and TNF-α, a major product of macrophages, modulate ZAG expression and secretion by human adipocytes. ZAG was produced primarily by adipocytes, and not by preadipocytes and macrophages. Incubation of preadipocytes with macrophage-conditioned medium for up to 12 days decreased ZAG mRNA and protein release, and the expression of adipogenic markers (PPARγ and C/EBPα). Adipocytes treated with macrophage-conditioned medium for 24 h displayed significant reductions in ZAG mRNA and release. Chronic TNF-α treatment let to significant decreases in ZAG expression and secretion, but marked upregulation of pro-inflammatory cytokines and chemokines (IL-6, leptin, IL-8, MCP-1 and RANTES) in adipocytes. These findings suggest that macrophage-associated inflammation may play a significant role in the downregulation of ZAG in adipose tissue in obesity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 325, Issues 1–2, 30 August 2010, Pages 135–142
نویسندگان
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