کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2199241 1099433 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystallins of the β/γ-superfamily mimic the effects of lens injury and promote axon regeneration
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Crystallins of the β/γ-superfamily mimic the effects of lens injury and promote axon regeneration
چکیده انگلیسی

Adult retinal ganglion cells (RGCs) can survive axotomy and regrow lengthy axons when exposed to lens injury (LI). The neuroprotective and axon-growth-promoting effects of LI have been attributed to an infiltration of activated macrophages into the inner eye and recently also to astrocyte-derived CNTF. The present work reveals that certain purified lens proteins (crystallins) cause the effects of LI. Intravitreal injections of β- or γ-crystallins, but not of α-crystallin, strongly enhanced axon regeneration from retinal explants in culture, within peripheral nerve grafts or the crushed optic nerve. Deposition of the effective crystallins within the vitreous body was also associated with an influx of circulating macrophages and an activation of retinal astrocytes, Müller cells, and resident microglia. Furthermore β-crystallin induced CNTF expression in retinal astrocytes and activation of CNTF's major downstream signaling pathway (JAK/STAT3) when intravitreally injected or added to the culture medium ex vivo. Consistently, in culture the addition of β- and γ-crystallins to the medium also increased axon regeneration from retinal explants. These results demonstrate that crystallins of the β/γ-superfamily are the lens-derived activators of cascades, which lead to axonal regeneration and suggest that their effects might be mediated by astrocyte-derived CNTF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 37, Issue 3, March 2008, Pages 471–479
نویسندگان
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