کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199297 | 1099436 | 2007 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: E-cadherin promotes retinal ganglion cell neurite outgrowth in a protein tyrosine phosphatase-mu-dependent manner E-cadherin promotes retinal ganglion cell neurite outgrowth in a protein tyrosine phosphatase-mu-dependent manner](/preview/png/2199297.png)
During development of the visual system, retinal ganglion cells (RGCs) require cell–cell adhesion molecules and extracellular matrix proteins for axon growth. In this study, we demonstrate that the classical cadherin, E-cadherin, is expressed in RGCs from E6 to E12 and promotes neurite outgrowth from all regions of the chick retina at E6, E8 and E10. E-cadherin is also expressed in the optic tectum. E-cadherin adhesion blocking antibodies specifically inhibit neurite outgrowth on an E-cadherin substrate. The receptor-type protein tyrosine phosphatase, PTPμ, associates with E-cadherin. In this manuscript, we demonstrate that antisense-mediated down-regulation of PTPμ, overexpression of catalytically inactive PTPμ and perturbation of endogenous PTPμ using a specific PTPμ inhibitor peptide results in a substantial reduction in neurite outgrowth on E-cadherin. Taken together, these findings demonstrate that E-cadherin is an important adhesion molecule for chick RGC neurite outgrowth and suggest that PTPμ expression and catalytic activity are required for outgrowth on an E-cadherin substrate.
Journal: Molecular and Cellular Neuroscience - Volume 34, Issue 3, March 2007, Pages 481–492