کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2199658 | 1099605 | 2016 | 4 صفحه PDF | دانلود رایگان |
• A novel, label-free fluorescence assay has been developed for the detection of Dam methyltransferase (MTase) activity.
• This method is highly selective and sensitive with a 0.1 U/mL limit of detection.
• Because no labeling was required, the method is simple and low cost.
We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in the range from 0.1 to 8.0 U/mL with a detection limit of 0.1 U/mL. And because no labeling with a fluorophore–quencher pair was required, it is simple and low cost. We envision that our novel fluorescent detection method for Dam MTase activity could be applied as a useful tool in biomedical research.
Journal: Molecular and Cellular Probes - Volume 30, Issue 2, April 2016, Pages 118–121