کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2199663 | 1099606 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Validation according to ISO/TS 12869:2012 of a molecular method for the isolation and quantification of Legionella spp. in water
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Validation according to ISO/TS 12869:2012 of a molecular method for the isolation and quantification of Legionella spp. in water Validation according to ISO/TS 12869:2012 of a molecular method for the isolation and quantification of Legionella spp. in water](/preview/png/2199663.png)
چکیده انگلیسی
The aim of the present work was to validate the performances of a new molecular method comprehensive of water sample filtration, DNA extraction and Real-Time PCR for the quantification of Legionella spp. in clear water samples, in accordance with the recent ISO Technical Specification 12869:2012. All criteria and requirements were verified considering inclusivity and exclusivity, check of the calibration function, limit of detection and limit of quantification, recovery calculation, robustness and uncertainty of the entire method. The performances were validated as all parameters resulted to be in compliance with values detailed by the above mentioned standard. The described method proved to be specific, sensitive, accurate and it has been fully validated according to ISO/TS 12869:2012. The possibility of using a validated molecular method will improve the reliability of the results making it a promising tool that should be used in addition to cultural analysis. Moreover, these findings make it particularly suitable for a relatively inexpensive screening of water samples, reducing the turnaround time and the workload.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 29, Issue 2, April 2015, Pages 86-91
Journal: Molecular and Cellular Probes - Volume 29, Issue 2, April 2015, Pages 86-91
نویسندگان
Enrica Omiccioli, Giuditta Fiorella Schiavano, Veronica Ceppetelli, Giulia Amagliani, Mauro Magnani, Giorgio Brandi,