کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2200784 | 1099971 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Botulinum toxin’s axonal transport from periphery to the spinal cord Botulinum toxin’s axonal transport from periphery to the spinal cord](/preview/png/2200784.png)
Axonal transport of enzymatically active botulinum toxin A (BTX-A) from periphery to the CNS has been described in facial and trigeminal nerve, leading to cleavage of synaptosomal-associated protein 25 (SNAP-25) in central nuclei. Aim of present study was to examine the existence of axonal transport of peripherally applied BTX-A to spinal cord via sciatic nerve.We employed BTX-A-cleaved SNAP-25 immunohistochemistry of lumbar spinal cord after intramuscular and subcutaneous hind limb injections, and intraneural BTX-A sciatic nerve injections. Truncated SNAP-25 in ipsilateral spinal cord ventral horns and dorsal horns appeared after single peripheral BTX-A administrations, even at low intramuscular dose applied (5 U/kg). Cleaved SNAP-25 appearance in the spinal cord after BTX-A injection into the sciatic nerve was prevented by proximal intrasciatic injection of colchicine (5 mM, 2 μl). Cleaved SNAP-25 in ventral horn, using choline-acetyltransferase (ChAT) double labeling, was localized within cholinergic neurons.These results extend the recent findings on BTX-A retrograde axonal transport in facial and trigeminal nerve. Appearance of truncated SNAP-25 in spinal cord following low-dose peripheral BTX-A suggest that the axonal transport of BTX-A occurs commonly following peripheral application.
Occurrence of cleaved SNAP-25 in ipsilateral lumbal ventral horn following botulinum toxin A (30 U/kg) subcutaneous injection into the rat hind paw.Figure optionsDownload as PowerPoint slideHighlights
► Peripheral BTX-A cleaves SNAP-25 in dorsal and ventral horn of the spinal cord.
► Axonal transport of BTX-A occurs at low intramuscular dose.
► BTX-A retrograde transport occurs via peripheral nerves and is microtubule-dependent.
► Truncated SNAP-25 is localized within cholinergic neurons of the ventral horn.
Journal: Neurochemistry International - Volume 61, Issue 2, July 2012, Pages 236–239