Changes and role of adrenoceptors in PC12 cells after phenylephrine administration and apoptosis induction

The present study addresses the hypothesis that adrenergic regulation modulates the effect of apoptosis. Therefore we studied, whether α1-adrenergic receptor's agonist phenylephrine (PE) can affect or induce apoptosis in rat pheochromocytoma (PC12) cells. We have shown that PE treatment did not increase level of the apoptosis, or level of the caspase 3 mRNA. When apoptosis was induced in the presence of PE, caspase 3 mRNA was significantly increased, while the percentage of apoptotic cells remained unchanged compared to apoptotic group without PE. During this process, α1D-, β2- and β3-adrenergic receptors (ARs) were upregulated. Since all these three types of ARs are differently localized in the cell, we assume that mutual communication of all three ARs is crucial to participate in this signaling and during development of apoptosis, some of these systems might translocate. Another important system in handling noradrenaline during apoptosis might be noradrenaline transporter (NET), since it was downregulated in apoptotic cells treated with PE, compared to untreated apoptotic cells. However, precise mechanism of mutual communication among all these systems remains to be elucidated.

Research highlights▶ Apoptosis in the presence of phenylephrine increased caspase 3 mRNA in PC12 cells. ▶ All types of beta adrenoceptors were upregulated by apoptosis, as well as alpha1D. ▶ During apoptosis, beta 2 AR translocates in the presence of alpha 1 D AR from nucleus to plasma membrane.