کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2203695 1100516 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative characterization of endothelial cell morphologies depending on shear stress in different blood vessels of domestic pigs using a focused ion beam and high resolution scanning electron microscopy (FIB-SEM)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Quantitative characterization of endothelial cell morphologies depending on shear stress in different blood vessels of domestic pigs using a focused ion beam and high resolution scanning electron microscopy (FIB-SEM)
چکیده انگلیسی


• We characterized statistically the EC morphologies depending on shear stress.
• The relationships between EC geometrical parameter and shear stress are exponential.
• EC aspect ratio increases exponentially with increasing shear stress.
• EC height decreases exponentially with increasing shear stress.

Microstructured surfaces mimicking the endothelial cell (EC) morphology is a new approach to improve the blood compatibility of synthetic vascular grafts. The ECs are capable of changing their shapes depending on different shear conditions. However, the quantitative correlation between EC morphology and shear stress has not yet been investigated statistically. The aim of this study was to quantitatively investigate the morphology of ECs in dependence on the shear stress. Blood flow rates in different types of natural blood vessels (carotid, renal, hepatic and iliac arteries) originated from domestic pigs were first measured in vivo to calculate the shear stresses. The EC morphologies were quantitatively characterized ex vivo by imaging with high resolution scanning electron microscopy (SEM) and cross-sectioning of the cells using a state-of-the-art focused ion beam (FIB). The relationships between EC geometrical parameters and shear stress were statistically analyzed and found to be exponential. ECs under high shear stress conditions had a longer length and narrower width, i.e. a higher aspect ratio, while the cell height was smaller compared to low shear conditions. Based on these results, suitable and valid geometrical parameters of microstructures mimicking EC can be derived for various shear conditions in synthetic vascular grafts to optimize blood compatibility.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tissue and Cell - Volume 47, Issue 2, April 2015, Pages 205–212
نویسندگان
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