Osteogenic commitment of human induced pluripotent stem cell-derived mesenchymal progenitor-like cells on biomimetic scaffolds

In this study, mesenchymal progenitor-like cells (MPLCs) were derived from human induced pluripotent stem cells (hiPSCs) by retinoic acid (RA) treatment. In particular, RA induced hiPSC–derived MPLCs were able to efficiently committed into osteogenic lineage cells. In addition, hiPSC–MPLCs were seeded onto three-dimensional biomimetic scaffolds composed of hydroxyapatite and poly(l-lactic acid)/poly(lactic-co-glycolide) for assessing osteoinductivity, and enhanced osteogenic activities were perceived in RA-treated cells in vitro and in vivo. In conclusion, addition of RA increased the osteogenic commitment potential of hiPSCs–MPLCs and further applications with biomimetic composite scaffolds showed a promising combination for bone tissue engineering applications.

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