کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
229666 465039 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid quantification of mirtazapine and desmethyl mirtazapine in human plasma by LC–ESI-MS/MS: Application to a bioequivalence study
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Rapid quantification of mirtazapine and desmethyl mirtazapine in human plasma by LC–ESI-MS/MS: Application to a bioequivalence study
چکیده انگلیسی

A high-throughout bioanalytical method based on a solid-phase extraction (SPE), and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis has been developed and validated for the quantification of mirtazapine (MRT) and desmethyl mirtazapine (DMRT) in heparinized human plasma. Plasma samples, without a drying and reconstitution step, were extracted by a simple SPE. The analytes and imipramine (internal standard, IS) chromatographed on a Betasil-C18 column. The total chromatographic run time was 1.8 min per sample. Response was a linear function of concentration in the range 0.1–100.0 ng/ml, with correlation coefficient ⩾0.9994. The assay has excellent characteristics and has been successfully applied to bioequivalence study samples for estimation of MRT and DMRT in healthy human subjects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Saudi Chemical Society - Volume 15, Issue 2, April 2011, Pages 145–153
نویسندگان
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