کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
231781 | 1427458 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Application of supercritical carbon dioxide (scCO2) for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica PCM 2266 Application of supercritical carbon dioxide (scCO2) for the extraction of lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica PCM 2266](/preview/png/231781.png)
The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four parameters, namely temperature, CO2 flow rate, pressure and co-solvent composition were analyzed. The best crude extract yields were achieved when the CO2 flow rate and temperature were kept high (10 g/min, 90 °C) and pure water was used as a co-solvent. Pressure had no statistically significant effect within the range of the study performed, whereas the other factors were relevant. The recovery of the extracted LPS by scCO2 was about 3.3% of the biomass used, while in the classical extraction method yield was less than 2%. All isolates were characterized by SDS-PAGE, by the spectra of the thiobarbituric acid reaction products and GLC–MS analysis.
Journal: The Journal of Supercritical Fluids - Volume 45, Issue 1, May 2008, Pages 51–56